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The sample (20 ml of stomach wash or urine or10- 20 gms of vomit) is taken in a conical flask. 50 ml of n-hexane is added. It is refluxed on a water bath for half- an-hour. After cooling, the liquid is filtered, mixed with 20 ml of n-hexane and taken in a separating funnel.
Headspace vial, flat top, flat bottom volume 10 mL, clear glass vial (flat top), flat bottom, O.D. × H × I.D. 20 mm × 54.5 mm × 12.5 mm, for Varian; find Supelco-854151 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
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Figure 3: GC/MS output for Air and Gas Sample, full Spectrum 27 Air in Fermentation Room, 1:50pm, 11/16/12 Fermentation Gas, 1:50pm, 11/16/12 Figure 4: GC/MS output for Air and Gas Sample, Low count focus, identification in Table 4 Table 4: Identification of Impurities in Off-Gas Sampling Time 1.5 2 4 6.0, 6.25, 6.5 7 7.5 8.5
Oct 13, 2005 · However, a customer has supplied a method where 22 mL vials are to be used. Would this 2 mL difference in volume be significant? The sample volume is approx' 2 mL. If it is significant, would it be reasonable to adjust the sample prep' to 91% of original the original volume, i.e. 1.82 mL instead of 2 mL (20 mL = 91% of 22 mL). Thanks in advance.