The Impact of Vial Dimension on Sample Volume and Injection Precision

Apr 2, 2019 · There are many variables such as (1) extraction time, (2) sample volume, (3) agitation, (4) temperature, and (5) sample matrix. As extraction time is increased, a plateau in peak area is reached. So, this represents the time required for the system to reach the equilibrium and is the optimized extraction time.

Nov 10, 2022 · Vials Use only Aijiren vials, the shape, and the material is optimized for maximizing instrument precision. Do not overfill the vials. Fill the vial up to 1/2 to 2/3 of the total volume. Experimental conditions Make sure that the same solvents were used between the runs. Keep the room temperature constant and under 25 degrees Celsius.

Jul 1, 2016 · Figure 2: Push-to-fill autosampler design: (a) withdrawing sample from sample vial; (b) needle in low-pressure seal filling sample loop; (c) valve rotated for injection of sample. Push-to-Fill. The push-to-fill autosampler is illustrated in Figure 2. Samples are placed in vials or 96-well plates and placed in a sample tray.

However, there are some effects related to increased injection volume and/ or concentration in HPLC/UHPLC which take place: the column can be overloaded if too much sample is injected onto the column; the height of the peak will increase with a larger injection volume; the peak width will broaden with an increase in injection volume.

The Two Principles of Injection. Sample injection in CE is basically done in two different ways: by pressure difference or by applying a voltage. Injecting by pressure difference is called hydrodynamic injection. The volume of sample injected depends not only on the injection time and pressure, but also on the BGE viscosity, the capillary

Aijiren system uses a sample loop of known size to collect the sample. The sampling steps for the valve and loop system are: 1 A needle probe punctures the vial. 2 The sampler pressurizes the vial with gas. See Figure 3. 3 After equilibration at pressure, the pressurized vial gases vent through the sample loop, filling the loop with sample.

needle. Fill 2/3 of the volume • If you use inserts make sure there are no airbubbles in them • Autosamplers with PEEK needle must have caps with slit septa • Never shake the vial. If you do there is going to be sample on the underside of the septum which gives carry over • Adjust the needle height according to the vial dimensions

The standard and thermostatted Aijiren Series autosamplers offer the possibility to inject volumes from 0.1 µl to 100 µl with high precision. However, for certain applications, such as semi-preparative HPLC or low con- centrated samples, higher sample vol- umes are required.

full-size rack holds 100 × 1.8 mL vials, while the two half-size racks provide space for 40 × 1.8 mL vials and 15 × 6 mL vials respectively. Any two half-size rack trays can be installed in the autosampler simultaneously. A specially designed sample-rack holding 100 × 1.8 mL vials is available for use with thermostatted autosamplers.

large volume injections. The SVI test report results in particles/ container while the LVI test report results in particles/mL. Table 1. SVI test and specifications Open/combine 10 or more units into a cleaned container for volume NLT 25 mL Degas (let stand, ultrasonic bath, or vacuum) Sample 4 times, disregard first, average last three

Aug 1, 2019 · autosamplers operate with the sampling needle as part of. the sample loop. It has two major instrumental setups—. with the metering device as part of the high-pressure flow. path (Figure 4a) or

The guidelines for sample injection volumes are as follows. If the injection volume is too large, the peak shape will become deformed, or the injection port will become dirty, leading to problems. Liquid samples: Approx. 1 to 2 µL Gas samples: Approx. 0.2 to 1 mL. Liquid samples become a gas at the stage of injection into the column.

The sample volume in the headspace vial has a large impact on the analytical results because it directly determines the beta. For a given gas-liquid equilibrium system, K and Co are constant and β is proportional to the concentration in the headspace gas. It is also said that when the sample volume Vs is increased, β is decreased, and Cg is

Reduce sample volume and/or concentration. Adjust gain. Inject smaller volume (e.g., 10 μL vs. 100 μL) or 1:10 or 1:100 dilution of sample. Change buffer strength, pH, or mobile phase composition. If necessary, raise column temperature or change column type. (Analysis of solute structure may help predict interaction.)

Oct 15, 2015 · Delay volumeComposition: accuracy, precision Repeatability of peak areas Dead volume Peak elution order Baseline: noise, drift and wander Flow: accuracy, precision Injection volume precision Linearity, dynamic range Carry over Wavelength: accuracy, precision Signal linearity Spectral resolution (DAD only) Column temperature accuracy