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Addgene: AAV Titration by qPCR 13/2/2019 · Seal plate with transparent film. Centrifuge at 3,000 rpm for 2 min to bring the sample to the bottom of the tube. Run the following protocol in your qPCR instrument using SYBR detection: 98°C 3 min / 98°C 15 sec / 58°C 30 sec / read plate/ repeat 39x from step 3 / melt curve. Example of plate set-up: 1. 2. 384-Well | Thermo Fisher Scientific Thermo Scientific™ 384-Well Conical Bottom Polypropylene Plates feature a lower binding capacity so proteins ...

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