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www.chromforum.org › viewtopicPerkinElmer Headspace TOGA – Chromatography Forum May 17, 2013 · Just as a trouble shooting exercise can you try a run with 7 ml of sample and see if it brings the sample peaks closer to the expected size. Or reduce the free volume in the standard vials by adding 15 ml (actual volume, not bulk volume) of glass beads or something. With that much sample you might also have a problem with equilibration. Peter chromachemie.co.in › headspace-vials-closuresVial...