Innovative Approaches to Improve Sample Recovery and Sensitivity in Chromatography

In high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase.

Jan 1, 1985 · Journal of Chromatography, 348 (1985) 123-129 Elsevier Science Publishers B.V., Amsterdam -- Printed in The Netherlands CHROM. 18 089 BRACKETING, A SIMPLE LOADING TECHNIQUE THAT INCREASES SAMPLE RECOVERY, IMPROVES RESOLUTION, AND INCREASES SEN- SITIVITY IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY G. GUINN* and D. L. HENDRIX Western Cotton Research Laboratory, USDA-ARS, 4135 East Broadway Road

Home / Pharma / Blog. Pharmaceutical Analysis. Separation Science Pharmaceutical offers free learning from the experts covering methods, applications, eBooks, webinars, eSeminars, expert forums, videos, tutorials and training for pharmaceutical analysis users of chromatography, mass spectrometry, spectroscopy, sample preparation and related analytical techniques.

Jul 1, 2018 · The study focused on comparison of on-line sample concentration techniques, i.e. sample stacking modes, applied in capillary electrophoresis in order to improve the detection of components of stamp pad inks so that destruction of analysed document could be minimized. In particular, such techniques were taken into consideration as: normal

This review discusses the main causes for the low sensitivity of chromatographic methods and aims to introduce different technologies for enhancing their sensitivity in the following aspects: (i) different pretreatment methods for improving clean-up efficiency and recovery; (ii) derivatization step for altering the chromatographic behavior of an

molecular mass and permits their detection and quantitation with extremely high sensitivity. High performance liquid chromatography (HPLC) facilitates the rapid, quantitative separation of compounds from each other and from the other constituents of complex mixtures or matrices. Used in tandem, the two techniques (usually referred to as LC-MS)

It is demonstrated that repeatability issues are mainly due to poor recovery of peptides from the sample vial. Problems are largely resolved by addition of an organic modifier to the sample vial to improve solubility of the peptides, but care needs to be taken not to lose peptides due to reduced affinity for reversed-phase materials.

Abstract: This talk describes recent innovations in particles, sorbents, surface chemistries, column/device formats, and reagents for HPLC and sample prep that address the need for higher throughput, better sensitivity, and greater resolution.

Oct 13, 2018 · Liquid chromatography separation followed by mass spectrometry detection, commonly called liquid chromatography-mass spectrometry (LC-MS) has become a standard in the analysis of many target compounds in biological samples. This approach has been highly successful in areas such as drug discovery secondary screenings, pharmacokinetics studies

Nov 11, 2016 · Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with

Jun 15, 2020 · Alternative strategies to improve the sensitivity of LFA, such as temperature-humidity 7 or fluidic control 8,9,10,11, probe-based 12,13,14 or enzyme-based signal enhancement 15,16, and sample

Oct 27, 2020 · For example, the light absorption properties of a chemical may be measured to determine its concentration in a sample. Calibration is the process of determining the sensitivity of an instrument to a particular analyte, or the relationship between the signal and the amount of analyte in a sample. Q1. As a group, devise a way in which you could

Jul 14, 2023 · Demystifying comprehensive two-dimensional gas chromatography (GC×GC) Robust, sensitive, and reliable ACCUTRACE Plus fuel marker analysis by 2-dimensional GC/MS. Get your GC-MS troubleshooting guide now! Improve Your Preventative GC Maintenance. Simplifying GC–MS sample preparation for product and material testing.

Jun 9, 2022 · The neuropeptide oxytocin (OT) regulates several peripheral and central functions and is a molecule of interest in psychiatric diseases such as autism spectrum disorder, schizophrenia, anxiety and depression. The study of OT in human serum samples is however hampered by inconsistent sample preparation and analysis as well as low endogenous blood concentration (1–10 pM). This results in

Jun 19, 2012 · The liner must accommodate the vaporized sample or the sample may back up or “backflash” into the carrier gas lines or other spaces. Inlet liner volume is very important when choosing the liner. The gaseous sample volume is a function of the type of solvent, the head pressure on the column, and the temperature of the inlet.